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學校名稱輔仁大學
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研究生(中)楊志堅
研究生(英)Yang Chih-Chien
論文名稱(中)D-胺基酸 Peptidyl-prolyl cis/trans 異構化的影響
論文名稱(英)D-Amino Acid Effects on Peptidyl-Prolyl cis/trans Isomerization
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指導教授(中)高華生
指導教授(英)Gao Heau-Shan
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學位類別碩士
畢業學年度94
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關鍵字(中)D-胺基酸 脯胺酸 cis/trans 異構化 胜肽
關鍵字(英)D-Amino Acid Proline cis/trans Isomerization Peptide
摘要(中)蛋白質是由胺基酸頭尾相接而成。胺基酸的性質會直接影響蛋白質的結構與功能,不同胺基酸的蛋白質結構具有不同的生理功能。而蛋白質與胜?的構形變化 (Conformations)與脯氨酸 (proline)有密切的關係,脯氨酸與其前面不同種類的胺基酸形成的胜?鍵對 Peptidyl-prolyl cis/trans 異構化的形成有特別影響。 本計畫為探討脯氨酸與其前一個D-胺基酸形成的D-Xaa-Pro鍵及脯氨酸與其前面的L-胺基酸形成的L-Xaa-Pro鍵,對Peptidyl-prolyl cis/trans 異構化差異性的影響,此研究將有助益於新藥的設計與開發。本研究計畫將使用固相胜?合成法(Solid-Phase Peptide Synthesis) 來製備一系列含有D-胺基酸類似物的脯氨酸胜?序列,並應用?H NMR核磁共振光譜分析儀進行系統性的D-胺基酸脯氨酸胜?序列對 Peptidyl-prolyl cis/trans 異構化的構形研究(Conformational Study)。
摘要(英)Proline residues have often been associated with conformational changes in peptides and proteins, because of their unique ability to undergo peptidyl-prolyl cis-trans isomerization about the peptide bond linking proline and the preceding amino acid residue. In order to examine the ability of D-amine acid residues preceding proline to stabilize a cis amide conformational relative to that observed with L-amino acid residues. We plan to prepare a series of D-amino acid analogs of linear peptides that contain an amide between D-amino acid and praline. The D-amino acid containing peptides will be prepared with the solid-phase peptide synthesis protocols. Conformation studies on the peptidyl-prolyl cis/trans isomerization of D-amino acid-proline peptides will be analyzed with ?H NMR. The result of these studies would be used for new drug development.
論文目次目錄 中文摘要 i 英文摘要 ii Abbreviations iii 謝 誌 v 目 錄 vi 圖 目 錄 viii 表 目 錄 xv 壹、 緒論 1 一、 氨基酸組成簡介 4 二、 D 型胺基酸的特性 10 貳、 原理 一、 固相胜?合成原理簡介 12 二、 核磁共振基本原理 16 三、 二維核磁共振光譜簡介 20 四、 二維核磁共振判定方法 25 參、 實驗部份 一、 實驗藥品 29 二、 胜?合成 32 三、 儀器設備 40 四、 核磁共振光譜測定 42 肆、 實驗結果與討論 一、胜?合成結果 43 二、胜?序列化學位移統計 46 三、二維核磁共振判定 49 四、Peptidyl-prolyl cis/trans 構形研究 52 伍、 結論 54 陸、 參考文獻 55
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