輔仁大學
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學校名稱輔仁大學
系所名稱化學系
舊系所名稱
學號492336085
研究生(中)林凡森
研究生(英)Fan Sen Lin
論文名稱(中)利用流道表面修飾及入口形狀設計以提升電泳晶片之效能
論文名稱(英)Performance Enhancement of Capillary Electrophoresis Chip by Surface modification and Design of Entrance Shape
其他題名
指導教授(中)陳壽椿
指導教授(英)Show-Chuen Chen
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國圖全文開放日期.2006.02.13
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學位類別碩士
畢業學年度94
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語文別中文
關鍵字(中)微電泳晶片、電化學偵測、神經傳導物質、聚合電解質、HPC、羥丙基纖維素、電場增益、擴散抑制
關鍵字(英)Electrophoresis microchip, Electrochemical detection, Neurotransmitters, Polyelectrolytes, HPC, Hydroxypropyl cellulose, Electri
摘要(中)微電泳晶片連接電化學偵測儀除了靈敏、快速、檢體及溶劑低消耗等優點外，微小化和批製也很容易。惟目前分離效率仍不及傳統毛細電泳，分離的速度也可再改善。本研究所發展的超級晶片系統(superchip)，負有兩大任務，即縮短樣品分離時間及提升訊號解析度；最終目的是希望能在數十秒內連續分離至少五個物種。本文所探討的參數乃因應這兩目標所做的前導研究。 據文獻報導，電泳入口若為沙漏形，會改變入口處的電阻值而獲得額外的電場強度，進而縮短分析時間。本實驗以神經傳導物質多巴胺與兒茶酚為樣品，以比較各型晶片的場增效應，及實驗過程的擴散效應。緩衝液中若摻入聚合電解質如羥丙基纖維素等，發現可以抑制溶質的擴散。最後發現以窄口雪莉酒杯型的入口設計，可以提高電場。而實驗過程中溶質擴散的問題，可以事先在流道中來回塗佈8 ppm的羥丙基纖維素(HPC)來克服。上述的電場增益及擴散抑制，可以同時提高速度及效率，以提供將來設計超級晶片時，所需的分離效率。
摘要(英)The advantages of sensitivity, speed, low consumption of sample and reagent, and the ease of integration--when annexing parts--with micro capillary electrophoresis are impressive. For one, it is surprisingly sensitive—at sub- orμM level—not too far below that by fluorescence but certainly superior to UV. However, its separation efficiency is inadequate for routine analysis, and besides its speed still has room for improvement. The name of superchip is coined to characterize a chip with high performances of speed, a few tens of seconds averaged for each sample, and separation efficiency with a peak capacity of five or more. This investigation serves the purpose of scouting the necessary traits for a superchip. The literature described the benefit of extra electric field strength by using an hourglass entrance for the separation channel, which, in turn, accerlerated migration. This investigation probes into the effects of entrance-shape induced field enhancement and diffusion suppression from repeated coatings of HPC, a polyelectrolyte, with the injection of dopamine and catechol. The chip performance can be maximized by shaping the entrance as a sherry wine glass to harness the benefit of field enhancement and by precoatings of 8 ppm HPC to suppress the degree of suppression. The sought-after versatility, sensitivity and speed for a superchip may emerge as more research is in progress.
論文目次中文摘要 i 英文摘要 ii 目錄 iii 圖目錄 v 表目錄 vii 第一章 緒論 1 1.1. 研究背景與目的 1 1.2. 毛細管電泳與微電泳晶片概論 3 1.2.1. 電泳技術發展史 3 1.2.2. 毛細管電泳的原理 5 1.3. 毛細管電泳基本分離模式 9 1.3.1. 毛細管區帶電泳法 9 1.3.2. 微胞電動力毛細管層析法 9 1.3.3. 毛細管等電電泳法 (Capillary Isotachophoresis, CITP) 10 1.3.4. 毛細管等電聚焦法 10 1.4. 微電泳晶片偵測方法 11 1.4.1. 雷射誘導螢光 (laser induced fluorescence, LIF) 11 1.4.2. 化學放光偵測法 (Chemiluminescence detection) 12 1.4.3. 質譜儀 (Mass Spectrometry) 13 1.4.4. 電化學偵測法 (Electrochemical detection) 14 1.4.5. 其他偵測方法 16 1.5. 毛細管電泳電化學偵測方法 17 1.5.1. 電位偵測法(potentiometric detection) 17 1.5.2. 電導度偵測法(conductivity detection) 18 1.5.3. 安培偵測法(amperometic detection) 19 1.6. 高速微電泳分離High-speed electrophoretic separation 21 第二章 實驗部份 23 2.1. 微電泳晶片之製作 23 2.1.1. 製程設備 23 2.1.2. 試劑與材料 24 2.1.3. 微電泳晶片製作流程 24 2.1.4. 感應電極基板製作 29 2.1.5. 高溫熔融接合 30 2.1.6. 微電泳晶片系統組裝 30 2.1.7. 外接式電極晶片改裝 31 2.2. 微電泳晶片之實驗 32 2.2.1. 儀器設備 32 2.2.2. 試劑 34 2.2.3. 實驗方法 36 2.2.4. 實驗操作 37 第三章 實驗結果與討論 40 3.1. 緩衝溶液流道入口形狀設計 40 3.1.1. 流道入口形狀對局部電場的影響 40 3.1.2. 晶片緩衝溶液入口形狀對樣品層析圖之影響 41 3.1.3. 晶片緩衝溶液入口形狀對樣品滯留時間之影響 44 3.2. 樣品擴散問題解決方法探討 45 3.2.1. 變更晶片緩衝溶液入口端到十字進樣區的距離 45 3.2.2. 聚合電解質羥丙基纖維素對樣品擴散抑制之研究 46 3.3. 樣品訊號解析度提升之研究 49 3.3.1. 晶片流道蝕刻時間對訊號解析度之影響 49 3.3.2. 長時間沖提下羥丙基纖維素對訊號之影響 51 3.3.3. 循環填充HPC對HPC安定性之探討 54 3.3.4. 電壓對提升解析度之影響 56 3.4. 緩衝溶液濃度對分離速度的影響 57 3.4.1. MES緩衝溶液濃度對樣品分離之影響 57 3.4.2. 不同濃度MES緩衝溶液添加同濃度HPC之比較 58 3.5. 多進樣系統的可行性調查及最佳化參數之研究 59 3.5.1. 多進樣分析系統 59 3.6. 整合超級晶片系統並運用於多進樣分析 63 第四章 結論 66 第五章 參考文獻 68
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