輔仁大學
學術資源網

記錄編號6271
狀態NC094FJU00065025
助教查核
索書號
學校名稱輔仁大學
系所名稱化學系
舊系所名稱
學號493336238
研究生(中)吳曉婷
研究生(英)Wu Hsiao-Ting
論文名稱(中)合成胜肽生物晶片應用於氣體感測之研究
論文名稱(英)Application of Synthetic Peptide in Biochip for Gas Sensing
其他題名
指導教授(中)高華生
指導教授(英)
校內全文開放日期不公開
校外全文開放日期不公開
全文不開放理由
電子全文送交國圖.同意
國圖全文開放日期.2012.07.30
檔案說明電子全文
電子全文01
學位類別碩士
畢業學年度94
出版年
語文別中文
關鍵字(中)合成胜肽
關鍵字(英)Synthetic Peptide
摘要(中)哺乳類動物皆具有嗅覺器官可以辨別出各種不同的氣味分子，當鼻腔上皮組織中的嗅覺受體 (olfactory receptor) 被氣味分子活化後，會促使 cAMP 的形成，進而開啟離子通道，並將嗅覺訊號傳遞至大腦。而嗅覺受體基因已經被選殖出，並且辨認出嗅覺受體蛋白的胺基酸序列。根據文獻資料顯示氣味分子與嗅覺受體蛋白的結合，可能主要與嗅覺受體蛋白的細胞外環狀結構 (extracellular loop) 及細胞膜通道區域 (transmembrane domain) 之特定胺基酸序列片段有關。 本研究利用固相胜?合成法，製備具有特定受體蛋白的胺基酸序列之合成胜?，進而將特定胺基酸序列片段聯結於壓電晶體訊號轉換元件 (piezoelectric transducer) 所製成的生物晶片上。探討仿嗅覺分子的合成胜?對氣體分子親和力的選擇性。此研究結果可提供具有特異性、選擇性辨認功能的仿嗅覺分子合成胜?，應用於氣味分子檢測工具的開發，亦可應用於醫藥、食品、環保、農藥、化妝品的樣品檢測。
摘要(英)The mammalian olfactory organs can distinguish different kind of odorant molecules. When olfactory receptor in the nasal epithelium is activated by odorant molecules can stimulate the formation of cAMP, then open ion channels, and transmit olfactory signals to the brain. The olfactory receptor gene has already been cloned, and the amino acid sequence of the olfactory receptor has been recognized. The literature suggested that the odorant binding domain in olfactory receptors might be related to the specific amino acid sequence of the extracellular loop and transmembrane domain. In this study, a series of synthetic peptides with specific amino acid sequence of olfactory receptor are prepared with the solid phase peptide synthesis method. The synthetic peptides are coated onto the surface of a piezoelectric electrode (PE). The PE biochip serves as a signal transducer to determine the binding affinity of synthetic peptides and odorants. The result of these studies can determine the binding specificity and selectivity of synthetic peptides with odorants. The result can use for further development of biochips for odorant sensing tools in medicine, food, environmental, pesticide and cosmetic.
論文目次中文摘要………………………………………………………………….i 英文摘要…………………………………………………………………ii Abbreviations………………………………………………………….iii 謝誌……………………………………………………………………...iv 目錄……………………………………………………………….……...v 圖目錄…………………………………………………………………...vi 表目錄…………………………………………………………………...ix 壹、緒論…………………………………………………………………..1 一、研究背景…………………………………………………………..1 二、固相胜?合成法…………………………………………………..4 三、生物感測器簡介…………………………………………………11 四、壓電晶體生物感測器簡介………………………………………16 貳、實驗部份............................................................................................21 一、實驗藥品…………………………………………………………21 二、儀器設備…………………………………………………………25 三、合成胜?製備……………………………………………………26 四、氣體偵測…………………………………………………………30 參、實驗結果與討論……………………………………………………34 一、合成胜?結果……………………………………………………34 二、目標氣體分子介紹………………………………………………38 三、氣體偵測結果……………………………………………………41 肆、結論…………………………………………………………………57 伍、參考文獻……………………………………………………………58 陸、附錄…………………………………………………………………61
參考文獻1. Buck, L.; Axel, R. 1991, A novel multigene family may encode odorant receptors: A molecular basis for recognition. Cell, 65, 175-187. 2. Pilpel, Y.; Lancet, D. 1999, The variable and conserved interfaces of modeled olfactory receptor proteins. Protein Science, 8, 969-977. 3. Hughes, A. L.; Hughes, M.K. 1993, Adaptive evolution in the olfactory gene family. J. Moi. Evol. 36, 249-254. 4. Freitag, F.; Ludwig, G.; Andreini, I.; Rossler, P.; Breer, H. 1998, Olfactory receptor in aquatic and terrestrial vertebrates. J. Comp. Physiol. A. 183, 635-650. 5. Ramachandran, G. N.; Mitra, A. k. 1976, An explanation for the rare occurrence of cis peptide units in proteins and polypeptides. J. Mol. Biol. 107, 85-92. 6. Nuzzo, R. G.; Zegzrski, B. R.; Dubois. L. H. 1987, Fundamental studies of chemisorption of organosulful compounds on Au (III). Implicatuon for molecular self-assembly on gold surface. J. Am. Chem. Soc. 109, 733-740. 7. Nuzzo, R. G.; Dubois. L. H.; Allara, D. L. 1990, Fundamental studies of microscopic wetting on organic surface. 1. Formatuon and structural characterization of a self-consistent series of polyfunctional organic monolayer. J. Am. Chem. Soc. 112, 558-569. 8. Merrifield, R. B. 1963, Solid phase peptide synthesis. J. Am. Chem. Soc. 85, 2149. 9. Merrifield, R. B. 1986, Solid phase peptide synthesis. Science, 232, 341-347. 10. Atherton, E.; Sheppard, R. C. 1989, Solid phase peptide synthesis: A practical approach. IRL Press, Oxford, England. 11. Larsen, B. D.; Holm, A. 1993, Expediting the Fmoc solid phase synthesis of long peptides through the application of dimethyl- oxazolidine dipeptides. J. Chem. Soc. Chem. Connun. 3, 369. 12. 童景炫. 1990, 胜?合成儀之原理. 科儀新知, 11, 19-31. 13. Kaiser, E.; Colescott, R. L.; Bossinger, C. D.; Cook, P. 1970, J. Anal. Biochem. 84, 595. 14. 吳宗正. 1999, 嗅覺生物晶片. 科學月刊, 30, 744-750. 15. Clark, L. C.; Lyons, C. 1962, Ann. N. Y. Acad. Sci. 102, 29-33. 16. Gopel, W. 1998, Chemical imaging: I. Concepts and visions for electronic and bioelectronic noses. Sensors and Actuators B, 25, 25-142. 17. Gopel, W.; Weimar, U. 1998, Chemical imaging: II. Trends in practical multiparameter sensor systems. Sensors and Actuators B, 52, 143-161. 18. 吳宗正. 1995, 生物感測器. 生物技術發展與應用, 18, 247-261. 19. 吳太光. 1997, 生物感測器. 生物技術發展與應用, 25, 461-480. 20. Scheller. F.; et.al. 1989, Research and development of biosensors. A Review. Analyst. 114, 653-662. 21. Czanderna, A. W. 1984, Applications of piezoelectric quartz crystal microbalance. Elseviser Science, New York. 22. 呂信賢. 2000, 生物電子鼻對臭異味分子的偵測及應用. 碩士論文, 國立東華大學生物技術研究所. 23. 施正雄. 2000, 壓電晶體化學感測器開發與應用. 科儀新知, 21, 60-72. 24. 黃錦城. 1997, 壓電晶體生物感測器之原理與應用. 食品工業月刊, 29, 8-16. 25. Sauerbrery, G, Z. 1959, Use of quartz crystal vibrator for weighting thin film on a microbalance. Z. Phys. 155, 206-210. 26. 曾明中、朱延和. 2003, 四兩撥千金-壓電石英微天秤. Bio Tech 生物科技. 3月號, 50-54. 27. Simenhoff, M. L.; Burke, J. F.; Saukkonen, J. J.; Ordinario, A. T.; Doty, R. 1977, Biochemical profile of uremic breath. N. Engl. J. Med. 297, 132-135. 28. Antony Manolis. 1983, The Diagnostic Potential of Breath Analysis. CLIN. CHEM. 29, 5-15.
論文頁數89
附註
全文點閱次數
資料建置時間
轉檔日期
全文檔存取記錄
異動記錄M admin Y2008.M7.D3 23:18 61.59.161.35