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學校名稱輔仁大學
系所名稱食品營養學系
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學號492446115
研究生(中)何皇滸
研究生(英)HUANG-HU, HO
論文名稱(中)蜂蜜酒釀造之研究
論文名稱(英)Studies on the Making of Honey Wine
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指導教授(中)陳雪娥
指導教授(英)Hsueh-err Chen
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學位類別碩士
畢業學年度94
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關鍵字(中)蜂蜜 蜂蜜酒 熟成 餐中酒 苦味
關鍵字(英)honey honey wine aging table wine bitter
摘要(中)蜂蜜是台灣養蜂人家的主要收入來源,多以稀釋後直接飲用,而缺乏其他的加工產品,雖有市售蜂蜜酒,但均為蒸餾酒型式,釀造酒很少見。蜂蜜酒有發酵時間長、產生苦味及不易澄清等問題,不易釀製成高品質的餐中酒。因此,本研究針對蜂蜜原料特性,探討發酵與熟陳條件,以釀製品質較佳的蜂蜜酒。 將蜂蜜以水稀釋至24°Brix,添加營養劑 ( Berthold之配方、實驗室改良配方、Fermaid KTM及Super food ),並以檸檬酸及酒石酸調整pH至3.2,接種菌? ( S. cerevisiae BCRC 21823、S. bayanus BCRC 21623、C?te des Blancs及Lalvin 71B-1122 ) 於20℃或25℃進行發酵,經16℃或25℃熟陳三個月後,探討蜂蜜來源、前處理、營養劑種類、菌種、發酵及熟成溫度等對蜂蜜酒品質之影響,並由品評找出適合蜂蜜酒的發酵條件。 菌種選擇方面,蜂蜜發酵醪分別接入四種菌?於20℃下進行發酵,新酒的成分不具差異性,熟成一個月後之品評結果以S. cerevisiae BCRC 21823及C?te des Blancs所釀造的蜂蜜酒風味品質較佳。選用此兩株酵母菌在不同溫度進行發酵,結果於25℃下的發酵速率較20℃快,經感官品評後以接種C?te des Blancs於25℃下釀造的蜂蜜酒具有較佳的風味品質。 前處理影響發酵速率,以加熱處理後再分離上清液之前處理組發酵最慢需25天,而其他前處理組15天即可發酵結束,但是加熱處理分離上清液釀製之蜂蜜酒澄清度高,而且風味上不具雜味,因此為適合的前處理操作。 不同營養劑的添加對發酵速率沒有顯著差異,但添加Super food能使酵母菌有效利用可發酵糖,轉換較多的酒精,酒精度可達13 % v/v,然而經品評後,添加自行改良配方之營養劑釀製之蜂蜜酒感官品質較佳。 不同蜂蜜來源之蜂蜜 ( 嘉義龍眼蜜、阿枝龍眼蜜、埔里龍眼蜜及埔里荔枝蜜 ),其發酵速率及感官品質亦有不同,以嘉義龍眼蜜發酵速率最快需20天,但埔里龍眼蜜需長達55天;將熟成三個月後之蜂蜜酒進行品評後,感官排序以埔里荔枝蜜所釀製之蜂蜜酒為最佳。 熟成溫度對於蜂蜜酒的成分影響不大,但隨著熟陳處理三個月,蜂蜜酒之澄清度上升且總酚含量下降。於25℃熟陳之褐變值明顯較16℃高,造成外觀上之品質較佳而非平淡無色。香氣方面,熟成溫度間不具顯著差異,但整體感則以25℃熟陳處理之蜂蜜酒較16℃佳。因此以埔里所產之荔枝蜂蜜配合最適的發酵條件,可釀造出品質較佳之蜂蜜酒。
摘要(英)Honey is the main income for beekeepers in Taiwan. Typically, honey consumption is based on watering down product, that other processed products are lacking. Although, certain honey wines are in the market, most of them are distilled, hardly made by brewing. Slow fermentation rate, bitter taste, as well as cloudiness development during storage are major concerns of honey wine, and those lead to a difficulty in brewing high quality table wines. Therefore, this study aimed to characterize various honeys, and to study the conditions of fermentation, and aging process for high quality products. Raw honey was diluted to 24°Brix by water. Mixture was added with nutrient additives such as additives used by Berthold, modified nutrient additives by our laboratory, Fermaid KTM and Super food, and the pH was then adjusted to 3.2 by citric acid and tartaric acid. The honey must was inoculated with yeast starters ( S. cerevisiae BCRC 21823, S. bayanus BCRC 21623, C?te des Blancs and Lalvin 71B-1122 ), and fermented at 20℃ or 25℃, respectively. The honey wine had been aged at 16℃ or 25℃ for 3 months. The influences of varieties of honey, pretreatments, nutrient additives, yeast strains, fermentation and aging temperatures, and on the quality of honey wine were studied. The conditions of fermentation for the optimal quality of honey wine were achieved by sensory evaluation in this study. Selection of yeast strains was accomplished by inoculating individual 4 strains in honey must, and fermented at 20℃. No significant difference was noticed among young wines, however, vinified by S. cerevisiae BCRC 21823 and C?te des Blancs wine samples had better sensory quality after one month aging. These two strains were then selected for the following fermentation, and the results revealed greater fermentation rate was related to process temperature, moreover, wine vinified by C?te des Blancs at 25℃was characterized with the best flavor quality from sensory evaluation. The fermentation rate was found in association with the different pretreatments. Separation of precipitate from heat treatment must result in the slowest fermentation rate of 25 days, but the others pretreatments took 15 days. The use of heat treatment and separate precipitate lead to the wine with greatest clarity and pure of flavor among pretreatments, thus this process was an adequate pretreatment for making honey wine. The inclusion of nutrient additives showed no significant difference in fermentation rate, but the addition of Super food allowed yeast strain to convert more fermentation sugar to alcohol up to 13 % (v/v). From the sensory test, the wine with nutrient additive formulated by our laboratory imparted better sensory characteristics. The fermentation rate and sensory quality were associated with the varieties of honey ( including longan honey form Chiayi, A-CHIH, Puli and lichee honey from Puli ), that the longan honey from Chiayi exhibited the fastest fermentation rate of 20 days, and those from Puli 55 days. The honey wine made of Puli lichee was ranked the best by sensory evaluation after 3 months aging. The aging temperatures didn’t affect the honey wine compositions, but the aging duration favored the clarity and caused the decrease of total phenol. The extent of browning during aging was greater at 25℃ than 16℃, what be beneficial to impart a better visual acceptance. The aging temperatures showed no significant difference in aroma perception, but overall sensory quality of honey wine aged at 25℃ was characterized better than those at 16℃. Therefore, high quality of honey wine is processed from the Puli lichee honey under the fermentation condition developed in this study.
論文目次大綱 頁次 第一章、前言…………………………………………………………1 第二章、文獻回顧……………………………………………………3 一、蜂蜜簡介…………………………………………………………3 二、蜂蜜酒釀造………………………………………………………3 三、影響蜂蜜釀造酒品質之因素……………………………………5 (1)蜂蜜原料……………………………………………………5 (2)原料之加熱前處理…………………………………………9 (3)酵母菌………………………………………………………12 a.酵母菌代謝產生的主要成分……………………………12 b.影響酵母菌生長之因素…………………………………16 i.營養需求………………………………………………16 ii.酵母菌種……………………………………………17 iii.菌?處理…………………………………………17 iv.發酵溫度……………………………………………19 (4)熟陳作用……………………………………………………22 (5)澄清處理……………………………………………………24 a.轉桶………………………………………………………24 b.安定處理…………………………………………………24 c.澄清劑……………………………………………………25 i.帶正電性澄清劑………………………………………25 ii.帶負電性澄清劑………………………………………27 d.過濾………………………………………………………27 第三章、材料與方法…………………………………………………28 一、實驗材料………………………………………………………28 二、儀器設備………………………………………………………29 三、實驗架構………………………………………………………30 四、蜂蜜酒的釀製流程……………………………………………31 五、物化分析………………………………………………………34 (一)、測定方法…………………………………………………34 (二)、釀酒過程中成分測定之項目……………………………46 六、感官品評分析…………………………………………………47 七、統計分析………………………………………………………49 第四章、結果與討論…………………………………………………50 一、菌種選擇………………………………………………………51 二、發酵溫度………………………………………………………61 三、不同前處理……………………………………………………70 四、不同營養劑……………………………………………………78 五、不同原料來源…………………………………………………86 第五章、結論…………………………………………………………100 第六章、參考文獻……………………………………………………102 表目錄 頁次 表一、台灣的主要及次要蜜源植物表…………………………………4 表二、蜂蜜之中國國家標準……………………………………………7 表三、市售蜂蜜之維生素及礦物質含量………………………………8 表四、市售的蜂蜜成分分析…………………………………………10 表五、市售蜂蜜產品中還原糖及羥甲基糠醛的含量………………11 表六、葡萄酒中各種酵母之酯類生成量……………………………14 表七、酵母菌對葡萄酒中含硫化合物生成之影響…………………18 表八、澄清劑之使用範圍……………………………………………26 表九、龍眼蜂蜜發酵醪之成分分析…………………………………51 表十、接種不同菌種之蜂蜜酒熟陳一個月後之成分分析…………58 表十一、接種不同菌種於不同溫度發酵熟陳一個月後之成分分 析……………………………………………………………67 表十二、接種不同菌種於不同溫度發酵熟陳一個月後之感官排 序……………………………………………………………68 表十三、不同前處理之蜂蜜酒熟陳一個月後之成分分析…………75 表十四、不同前處理之蜂蜜酒熟陳一個月後之感官排序…………77 表十五、添加不同營養劑之蜂蜜酒熟陳一個月後之成分分析……84 表十六、添加不同營養劑之蜂蜜酒熟陳一個月後之感官分析……85 表十七、不同蜂蜜來源之組成………………………………………87 表十八、蜂蜜酒熟成過程中pH、可滴定酸、揮發性酸及酒精度之 變化…………………………………………………………92 表十九、蜂蜜酒熟成過程中總酚及澄清度之變化…………………94 表二十、蜂蜜酒熟成過程中L, a, b及褐變值之變化……………95 表二十一、不同蜂蜜來源之蜂蜜酒於16℃熟陳三個月之感官排 序…………………………………………………………96 表二十二、不同蜂蜜來源之蜂蜜酒於25℃熟陳三個月之感官排 序…………………………………………………………97 表二十三、比較不同熟陳溫度之荔枝蜂蜜酒熟陳三個月之感官排 序…………………………………………………………99 圖目錄 頁次 圖一、市售蜂蜜之生產流程…………………………………………6 圖二、酵母菌在發酵期間產生之主要風味成分的產生途徑………13 圖三、比較接種10 %及0.4 %菌?於苜宿蜜的發酵速率…………20 圖四、不同溫度之發酵過程中菌數的變化…………………………21 圖五、不同溫度對苜蓿蜂蜜發酵速率之影響………………………23 圖六、蜂蜜酒製造流程圖……………………………………………30 圖七、20℃發酵期間不同菌種對蜂蜜酒醪發酵速率之影響………53 圖八、20℃發酵期間不同菌種對蜂蜜酒醪pH之影響………………54 圖九、20℃發酵期間不同菌種對蜂蜜酒醪可滴定酸之影響………55 圖十、20℃發酵期間不同菌種對蜂蜜酒醪揮發性酸之影響………56 圖十一、 接種不同酵母菌發酵之蜂蜜酒經熟陳一個月後之平均 感官排序……………………………………………………60 圖十二、不同菌種及發酵溫度對蜂蜜酒醪發酵速率之影 響……………………………………………………………62 圖十三、不同菌種及發酵溫度對蜂蜜發酵期間揮發性酸 之影響………………………………………………………63 圖十四、不同菌種及發酵溫度對蜂蜜發酵期間pH之影 響……………………………………………………………65 圖十五、不同菌種及發酵溫度對蜂蜜發酵期間可滴定酸 之影響………………………………………………………66 圖十六、25℃發酵期間不同前處理對蜂蜜酒醪發酵速率之影響…71 圖十七、25℃發酵期間不同前處理對蜂蜜酒醪pH之影響…………72 圖十八、25℃發酵期間不同前處理對蜂蜜酒醪可滴定酸之影響…73 圖十九、25℃發酵期間不同前處理對蜂蜜酒醪揮發性酸之影響…74 圖二十、25℃發酵期間不同營養劑對蜂蜜酒醪發酵速率之影響…79 圖二十一、 25℃發酵期間不同營養劑對蜂蜜酒醪可滴定酸之影 響…………………………………………………………80 圖二十二、 25℃發酵期間不同營養劑對蜂蜜酒醪pH之影 響…………………………………………………………81 圖二十三、 25℃發酵期間不同營養劑對蜂蜜酒醪揮發性酸 之影響……………………………………………………82 圖二十四、 不同蜂蜜來源之蜂蜜發酵醪於25℃發酵期間發酵 速率之變化………………………………………………88 圖二十五、 不同蜂蜜來源之蜂蜜發酵醪於25℃發酵期間pH 之變化……………………………………………………89 圖二十六、 不同蜂蜜來源之蜂蜜發酵醪於25℃發酵期間可滴 定酸之變化………………………………………………90 圖二十七、 不同蜂蜜來源之蜂蜜發酵醪於25℃發酵期間揮發 性酸之變化………………………………………………91 附錄 頁次 附錄一、20℃下比重與酒精度對照表………………………………114 附錄二、比重及抽出物含量之對照圖………………………………115 附錄三、還原糖及總糖滴定計算對照表……………………………116 附錄四、蜂蜜酒差異性排序法品評問卷……………………………117
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