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學校名稱輔仁大學
系所名稱食品營養學系
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學號492446191
研究生(中)蔡明娟
研究生(英)Ming-Chuan Tsai
論文名稱(中)運動後不同升糖指數碳水化合物補充對人體骨骼肌GLUT4蛋白質表現之影響
論文名稱(英)Effects of different GIs carbohydrate supplementation after exercise on GLUT4 protein expression in human skeletal muscle
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指導教授(中)李寧遠
指導教授(英)
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關鍵字(中)運動、葡萄糖轉運子4號、升糖指數、西方點墨法
關鍵字(英)Exercise, GLUT4, glycemic index, western blotting
摘要(中)葡萄糖轉運子4號(Glucose transporter 4, GLUT4)為肌肉中肝醣再合成作用中的重要因子之一。研究發現,老鼠及人體骨骼肌內GLUT4 mRNA及蛋白質表現量會受到運動或碳水化合物補充調節。本實驗目的在探討運動後立即補充不同升糖指數(Glycemic index, GI)碳水化合物對GLUT4蛋白質表現之影響。18名非運動員之大學生(年齡21.3±1.46歲,身高173.1±5.33公分,體重70.21±7.34公斤,攝氧量峰值43.65±10.58 毫升/公斤/分鐘),在單一次75%最大攝氧量峰值腳踏車運動60分鐘後,隨機分成3組,分別給予禁食、高GI碳水化合物及低GI碳水化合物三種不同試驗。運動後及運動後3小時各進行一次肌肉穿刺取得肌肉樣本,以西方墨點法(Western Blotting)技術定量葡萄轉運子的蛋白質表現,收集血液樣本測定血液中葡萄糖及胰島素的濃度。不同GI組血糖反應曲線下面積(Area under curve, AUC)沒有達到顯著差異,血糖及胰島素濃度3組間具有統計上的顯著差異,高GI組胰島素曲線下面積高於低GI組(p<0.05)。高GI組GLUT4蛋白質表現顯著高於低GI組及禁食組。這次結果顯示,運動後恢復期,補充高GI碳水化合物飲食能增加胰島素的分泌,並促進GLUT4蛋白質表現,推測胰島素的作用對於GLUT4蛋白質的表現應是調節的角色而非刺激物。
摘要(英)Glucose transporter subtype 4 (GLUT4) is an important factor for glycogen resynthesis in muscle. The mRNA and protein level of GLUT4 can be regulated with exercise or carbohydrate supplementation in rat and human skeletal muscle. The purpose of this study is to investigate the effect of different glycemic index (GI) carbohydrate supplementation after exercise on GLUT4 protein expression in human skeletal muscle. Eighteen untrained subjects(age 21.3±1.46 yr, height 173.1±5.33cm, weight 70.21±7.34 kg, VO2 peak 43.65±10.58 ml/kg/min) were asked to take a single bout of cycle ergometer at 75% peak oxygen consumption for 60 minutes. Subjects were randomly assigned to one of three treatment groups after exercise: fasting, high GI carbohydrate (HGI) supplement or low GI (LGI) carbohydrate supplement. Biopsies were performed on the deep portion of the vastus lateralis muscle of all subjects immediately after exercise and 3 hours after the carbohydrate ingestion. Protein expressions of GLUT4 are showed quantitatively with Western Blotting technique. Blood samples are collected immediately after the exercise and every 30 minutes thereafter, up to 3 hours. Blood samples were collected for glucose and insulin analysis. The blood area under curve (AUC) is not different between HGI and LGI groups. Concentrations of blood glucose and insulin are significant different from three groups (p<0.05). Insulin AUC is significant higher in HGI group than in LGI and fasting groups. GLUT4 protein content in HGI group is significant increase above LGI and fasting trails. The results of this study suggest that HGI carbohydrate supplement after exercise can enhance GLUT4 protein expression and increase insulin secretion during recovery period. We propose that the insulin may play a role of mediator than stimulator for GLUT4 protein.
論文目次中文摘要------------------------------i 英文摘要-------------------------------iii 目錄-----------------------------------v 表目錄---------------------------------viii 圖目錄---------------------------------ix 第一章 緒論-----------------------------1 第一節 問題背景--------------------------1 第二節 研究目的--------------------------3 第三節 研究假說--------------------------4 第四節 研究範圍限制----------------------- 4 第五節 名詞操作型定義--------------------- 4 第二章 文獻回顧-------------------------- 6 第一節 肝醣合成作用----------------------- 6 第二節 運動後恢復期之肝醣合成作用----------- 8 第三節 運動後恢復期碳水化合物之影響--------- 9 第四節 升糖指數碳水化合物對運動之影響------- 12 第五節 葡萄糖轉運子(Glucose transporter, GLUT)---15 第三章 材料與方法------------------------- 20 第一節 受試對象---------------------------20 第二節 實驗設計與流程----------------------20 (一) 實驗處理-----------------------------20 (二) 測試食物之GI值------------------------21 (三) 測試攝氧峰值(VO2 peak)之運動Protocol---24 (四) 受試者注意事項-------------------------25 (五) 實驗流程------------------------------26 第三節 實驗方法-----------------------------27 (一) 肌肉穿刺------------------------------27 (二) 血液採集及處理-------------------------27 (三) 葡萄糖轉運子蛋白質分析------------------28 (四) 血液分析------------------------------29 第四節 統計方法-----------------------------30 第四章 結果---------------------------------31 第一節 受試者基本資料------------------------31 第二節 HGI及LGI組平均三大營養素及熱量攝取------31 第三節 血糖反應------------------------------32 第四節 胰島素反應----------------------------32 第五節 GLUT4蛋白質表現-----------------------33 第五章 討論---------------------------------41 第一節 主要發現------------------------------41 第二節 血液生化值之反應-----------------------41 第三節 單一運動對GLUT4蛋白質表現之影響---------43 第四節 運動後補充GI碳水化合物GLUT4蛋白質表現之影響-- 45 第五節 結論----------------------------------49 參考文獻-------------------------------------50 附錄一、GLUT4西方點墨法(Western Blooting) ----67 附錄二、升糖指數碳水化合物飲食內容--------------74 附錄三、升糖指數飲食詳細營養成分表--------------75
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